Point of Care Diagnostics (Lab on Chip)

The group is developing lab-on-chip devices for (mostly) nucleic acid – based and antigen/antibody-based, on-site detection of pathogens in body fluids and in food and for genotyping.

pointofcare

A cassette for the processing of nucleic acids, PCR, and lateral flow-based detection

POC

Keywords: Lab on Chip, LOC, point of care, diagnostics, POC, nucleic acids, enzymatic amplification, isothermal amplification, uninstrumented devices, self heating, exothermic

PUBLICATIONS

  • Qian, S. and Bau, H., H., 2003, A Mathematical Model of Lateral Flow Bio-Reactions Applied to Sandwich Assays, Analytical Biochemistry, 322, 89-98.
  • Qian S., and Bau, H., H., 2004, Analysis of Lateral Flow Bio-detectors: Competitive Format, Analytical Biochemistry 326, 211–224.
  • Chen, Z., Qian, S., Abrams, W., R., Malamud, D., and Bau, H., H., 2004, Thermosyphon-based PCR Reactor: Experiment and Modeling, Analytical Chemistry, 76, 3707-3715.
  • Qian, S. and Bau, H., H., 2005, Magneto-hydrodynamic Stirrer for Stationary and Moving Fluids, Sensors and Actuators B: Chemical, 106, 859-870
  • Qian, S. Burger, R., and Bau, H., H., 2005, Analysis of Sedimentation Biodetectors, Chemical Engineering Science, 60, 2585 – 2598.
  • Malamud, D., H. Bau, S. Niedbala, P. Corstjens, 2005, Point Detection of Pathogens in Oral Samples, Adv Dent Res 18:12-16.
  • Wang, J., Chen, Z., Qian S., and Bau, H. H., 2005, Thermally-Actuated, Phase-Change Flow Control for Microfluidic Systems, Lab on Chip, 5, 1277 – 1285.
  • Wang, J., Chen, Z., Mauk, M., Hong, K-S, Li, M., Yang, S., and Bau, H., H., 2005, Self-Actuated, Thermo-Responsive Hydrogel Valves for Lab on a Chip, Biomedical Microdevices 7 (4), 313-322.
  • Z. Chen, J. Wang, M. G. Mauk, H. H. Bau, C. Davis, G. Tong, F. Winslow, W. R. Abrams, D. Malamud, 2005, On Chip PCR Amplification with Integrated Lateral Flow Detection, no. 0240, µTAS 2005, Boston, Massachusetts, USA, October 9¬13th, 2005, 1297-1299.
  • Z. Chen, P.L.A.M. Corstjens, M. Zuiderwijk, J. Wang, M. G. Mauk, H. H. Bau, W. R. Abrams, D. Malamud, 2005, Phosphor Reporter Particle-Based Disposable Device for the Rapid Detection of HIV at the Point of Care, no. 0241, µTAS 2005, Boston, Massachusetts, USA, October 9¬13th, 2005, 791-793.
  • Wang, J., Mauk, M.G., Chen, Z., Bau, H.H., 2005, “A disposable Microfluidic Device for Cell Lysis and DNA Isolation”, no. 0345, Proceedings of μTAS 2005, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Boston, Massachusetts, USA, K.F. Jensen, J. Han, D. J. Harrison, and J. Voldman (editors) October 9th ¬ 13th, 2005, pp 289-291.
  • Wang, J., Chen, Z., Corstjens, P. L. A. M., Mauk, M., and Bau, H. H., 2006, A Disposable Microfluidic Cassette for DNA Amplification and Detection, Lab on Chip, 6, 46-53
  • Ziober, B., Mauk, M., Chen, Z. and Bau, H., H., 2008, Lab-on-a-Chip for Oral Cancer Screening and Diagnosis, Head & Neck , 30, (1), 111-121
  • Abrams, W. R., C. A. Barber, K. McCann, G. Tong, Z. Y. Chen, M. G. Mauk, J. Wang, A. Volkov, P. Bourdelle, P. L. A. M. Corstjens, M. Zuiderwijk, K. Kardos, S. Li, H. J. Tanke, R. S. Niedbala, D. Malamud and H. H. Bau (2007). “Development of a Microfluidic Device for Detection of Pathogens in Oral Samples Using Upconverting Phosphor Technology (UPT).” Annals of the New York Academy of Science 1098: 375-388.
  • Chen, Z., M. G. Mauk, J. Wang, W. R. Abrams, P. L. A. M. Corstjens, R. S. Niedbala, D. Malamud and Haim H.Bau (2007). “A Microfluidic System for Saliva-Based Detection of Infectious Diseases.” Annals of the New York Academy of Science 1098: 429-436.
  • Corstjens, P. L. A. M., Z. Chen, M. Zuiderwijk, H. H. Bau, W. R. Abrams, Daniel Malamud, R. S. Niedbala and H. J. Tanke (2007). “Rapid Assay Format for Multiplex Detection of Humoral Immune Responses to Infectious Disease Pathogens (HIV, HCV, and TB).” Annals of the New York Academy of Science 1098: 437-445.
  • Mauk, M. G., B. L. Ziober, Z. Chen, J. A. Thompson and Haim H. Bau (2007). “Lab-on-a-Chip Technologies for Oral-Based Cancer Screening and Diagnostics: Capabilities, Issues, and Prospects.” Annals of the New York Academy of Science 1098: 467.
  • Byun, D., Lee, Y., Tran, S. B. Q., Nugyen, V., Kim, S., Park, B., Lee, S., Inamdar, N., and Bau, H. H., 2008, Electrospray on Superhydrophobic Nozzles Treated with Argon and Oxygen Plasma, APPLIED PHYSICS LETTERS 92, 093507
  • Kim, J., Byun, D., Mauk, M., G., and Bau, H., H., 2009, A Disposable, Self-Contained PCR Chip, Lab on Chip 9, 606-612 (DOI 10.1039/b807915c). The paper was featured in an editorial in Analytical Chemistry, R. Mukhopadhyay, 2009, PCR to Go, January 8, 2009
  • Liu, C., Qiu, X., Ongagna, S., Chen, D., Chen, Z., Abrams, W., Malamud, D., Corstjens, P., and Bau, H., H., 2009, A Self-Contained, Timer-Actuated, Immunoassay Cassette for Detecting Molecular Markers in Oral Fluids, Lab on Chip, 2009, 9, 768 – 776: DOI:10.1039/B814322F.
  • Xianbo Qiu, Jason A. Thompson, Zongyuan Chen, Changchun Liu, Dafeng Chen, Sudhir Ramprasad, Michael G. Mauk, Serge Ongagna, Cheryl Barber, William R. Abrams, Daniel Malamud, Paul L.A.M. Corstjens, and Haim H. Bau, 2009, Finger-Actuated, Self-Contained Immunoassay Cassettes, Biomedical Microdevices 11 (6) 1175-1186.
  • Liu, C., Schrlau, M., Bau, H. H., 2009, Single bead-based electrochemical biosensor, Biosensors and Bioelectronics 25, 809-814.
  • Kim, J., Chen, D., and Bau, H. H., 2009, An Automated, Pre-programmed, Multiplexed, Hydraulic Microvalve, Lab on Chip 9, 3594-3598, DOI: 10.1039/B914865E.
  • Thompson, J., A., and Bau, H., H., 2010, Microfluidic, Bead-Based Assay: Theory and Experiments, Journal of Chromatography B 878, 228–236 (JCB-09-542R1)
  • D Chen, M. Mauk, X. Qiu, C. Liu, J. Kim, S. Ramprasad, S. Ongagna, W. R. Abrams, D. Malamud, P. L.A.M. Corstjens, and Haim H. Bau, 2010, “An Integrated, Self-Contained Microfluidic Cassette for Isolation, Amplification, and Detection of Nucleic Acids” Biomedical Microdevices 12 (4), 705-719. DOI 10.1007/s10544-010-9423-4.
  • Jitae Kim, Michael Mauk, Dafeng Chen, Xianbo Qiu, Jungkyu Kim, Bruce Gale, and Haim H. Bau, 2010, “A PCR REACTOR WITH AN INTEGRATED ALUMINA MEMBRANE FOR NUCLEIC ACID ISOLATION,” Analyst 135, 2408 – 2414 (DOI:10.1039/C0AN00288G)
  • Xianbo Qiu, Michael G. Mauk, Dafeng Chen, Changchun Liu, and H. H. Bau, 2010, “A Large Volume, Portable, Real-Time PCR Reactor,” Lab on a Chip, 10, 3170-3177. DOI:10.1039/C0LC00038H.
  • Thompson, J., Du, X., Grogan, J., M., Schrlau, M., G., and Bau, H., H., 2010, Polymeric microbead arrays for microfluidic applications, Journal of Micromechanics and Microengineering 20, 115017.
  • C. Liu, E. Geva, M. Mauk, X. Qiu, W. R. Abrams, D. Malamud, K. Curtis, S. M. Owen, and H. H. Bau, 2011, An Isothermal Amplification Reactor with an Integrated Isolation Membrane for Point-of-Care Detection of Infectious Diseases, Analyst 2011, 136, 2069-2076 DOI:10.1039/C1AN00007A. [The article was featured in Erica Wise, DNA Detection for Rapid HIV Diagnosis, RSC Chemistry World, April 01, 2011, http://www.rsc.org/chemistryworld/News/2011/April/01041101.asp]
  • Liu, C., Thompson, J., and Bau, H. H., 2011, A membrane-based, high-efficiency, microfluidic debubbler, Lab on Chip 11, 1688-1693, DOI:10.1039/C1LC20089E.
  • Liu, C., Mauk, M., Bau, H. H., 2011, A Disposable, Integrated Loop-Mediated Isothermal Amplification Cassette with Thermally-Actuated Valves, Microfluidics and Nanofluidics 11, 209-220 (DOI: 10.1007/s10404-011-0788-3).
  • Hart, R. W., Mauk, M., G., Liu, C., Qiu, X., Thompson, J., A., Chen, D., Malamud, D., Abrams, W., R., and Bau, H., H., 2011, Point of Care Oral-Based Diagnostics, Oral Diseases, 17, 745–752. DOI: 10.1111/j.1601-0825.2011.01808.x.
  • Thompson, J., and Bau, H., H., 2011, Pulsating Bead-Based Assay, Analytical Chemistry, Anal. Chem., 2011, 83 (8), pp 2858–2861, DOI: 10.1021/ac200410v.
  • Qiu, X., Chen, D., Liu, C., Mauk, M., G., Kientz, T., and Bau, H. H., 2011, A Portable, Integrated Analyzer for Microfluidic – Based Molecular Analysis, Biomedical Microdevices 13:809–817 (DOI: 10.1007/s10544-011-9551-5)
  • Liu, C., Mauk, M., Hart, R., Qiu, X., and Bau, H., H., 2011, Self Heating Cartridge for Molecular Diagnostics, Lab on Chip 11, 2686-2692. DOI: 10.1039/C1LC20345B [The article was featured by M. Smith, 2011, HOT: A self-heating cartridge for molecular diagnostics, Lab on Chip Blog, http://blogs.rsc.org/lc/2011/07/21/hot-a-self-heating-cartridge-for-molecular-diagnostics/] [Among the ten most downloaded papers in July 2011]
  • Qiu, X., Liu, C., Mauk, M., Hart, R., Chen, D., Qiu, J., Kientz, T., Fiene, J., and Bau, H., H., 2011, A Portable Analyzer for Pouch-Actuated Immunoassay Cassettes, Sensors and Actuators B, 160 1529–1535.
  • M. G. Mauk, Changchun Liu, Paul L.A.M. Corstjens, and Haim H. Bau, 2011, Streamlining POC microfluidic molecular diagnostics, IVD Technology 17 (6), 21-28.
  • Thompson, J., and Bau, H., H., 2012, Porous Bead-Based Microfluidic Assay: Theory and Experiments, Micro and Nano Fluidics 12 (1), 625-637.
  • Liu, C., Mauk, M., G., Hart, R., Bonizzoni, M., Yan, G., and Bau, H., H., 2012, A Low-Cost Microfluidic Chip for rapid Genotyping of Malaria-Transmitting Mosquitoes, PLoS ONE, 7(8):e42222.doi:10.1371/journal.pone.0042222 2012-05-24.